Composite

Part:BBa_K3332101:Design

Designed by: Meihao Ou   Group: iGEM20_XMU-China   (2020-10-27)


J23100-RBS-phnJ_mut21-B0015-J23100-RBS-phnE1-RBS-phnE2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1057
    Illegal NheI site found at 2583
    Illegal NheI site found at 2606
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3234
    Illegal AgeI site found at 2209
    Illegal AgeI site found at 2905
    Illegal AgeI site found at 3412
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Construct from existed parts according to Standard BioBrick Assembly


References